albA [2019-03-12 19:11:34]

radical S-adenosylmethionine enzyme, antilisterial bacteriocin (subtilosin) production

locus: BSU37370

pI: 6.20

mw: 51.35 kDa

protein length: 448 aa

gene length: 1347 bp

function: antilisterial bacteriocin (subtilosin) production

product: radical S-adenosylmethionine enzyme

essential: no

synonyms: ywiA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 3,836,323 → 3,837,669

The protein

Catalyzed reaction/ biological activity

• catalyzes thioether bond formation in subtilosin A [Pubmed|22366720]

[SW|Cofactors]

• contains two Fe-S clusters [Pubmed|22366720]

Structure

• [PDB|6EFN] ([protein|F981C605C652D1A4429579A5F24608CE7F570834|SkfB],corresponds to aa 109 ... 382, 32% identity)

Expression and Regulation

Operons

• genes: [gene|8CBBEC47A5CC3EAE73BE68E68417C5F2A4DA5C23|sboA]-[gene|83AAFAA187B4FCEE24B4EA2DD4935425A3B48DAA|sboX]-[gene|DEDA6F03386F360F6C0B2CC5F09DF3C9D85173F1|albA]-[gene|D399B25AE22ECB6C7ACDCFF4DCE4D9D908184E75|albB]-[gene|19CC58F5ABADAD45BA55D88BBD77B586AA5D85FE|albC]-[gene|DAD8308DF72E648610D1A12BC92063E1550CB1B4|albD]-[gene|A474F01AA4AD859963E39CD528BC42FF43A72433|albE]-[gene|D03FB4EAEFBC25A5B0459CF464BFADF1FC7620B2|albF]-[gene|55A34610EF7AA5E515DE3DCDA333ED3A42B0EF08|albG]
  description: [Pubmed|10572140]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10572140], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|10809710], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|10572140,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • expressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [Pubmed|10809710]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• MGNA-B263 (ywiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1262 NBRP B. subtilis, Japan]
• BKE37370 (Δ[gene|DEDA6F03386F360F6C0B2CC5F09DF3C9D85173F1|albA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAATACCATCCCCTATAC, downstream forward: _UP4_CAGCTTATTTAGGAGGGAAA
• BKK37370 (Δ[gene|DEDA6F03386F360F6C0B2CC5F09DF3C9D85173F1|albA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAATACCATCCCCTATAC, downstream forward: _UP4_CAGCTTATTTAGGAGGGAAA

References

• 10809709,10572140,15743949,10809710,10572140,17720793,17190806